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Overview of EM radiation

EM radiation & UV


electronic transition

Analysis of molecular data-spectrophotometric techniques-UV

Energy, wavelength and frequency of EM and applications of UV and rotational/vibrational states

Dual beam UV & molar extinctions & spectral variations comes from closely related small organic compounds

Red and blue shift-UV spectral shift; electronic transitions with examples

peptide bond formation & protein chromophores

chromophores:carotenoids, porphyrins in proteins and genetic materials

Factors affecting UV absorption/red and blue shift

Calculation of transmittance & Determination of absorbance & fluorescence spectroscopy

IC and ISC transitions and fluorophores

Fluorescence spectroscopy: 90 and 45 degree geometry

intrinsic and extrinsic fluorophores/FRET

BRET & CD spectroscopy/ORD

CD spectra analysis

IR spectroscopy

Analysis of IR spectra/amide vibrations

Analysis of IR spectra/theoretical simualtions

Raman spectroscopy

spectroscopic techniques: structure and interaction

ITC data analysis

X-ray crystallography: molecular structure analysis

Bragg's law /diffraction pattern/nature of crystals


factors that affect crystallization

Unit cells/asymmetric units

symmetries in crystal & phase problem in X-ray crystallography

symmetry equivalents and space groups

space group-orthorhombic system/Mathews coefficients

structure factor and electron density calculation

ESI-MS problems

problems revision

mass spec-problems

Pre-recorded videos (folders)